Restriction fragment length polymorphism (RFLP)
- Pronunciation
- /ree-STRIK-shun FRAG-ment LENGth pol-ee-MOR-fiz-um/
- Category
- Physiology
- Singular
- restriction fragment length polymorphism
- Plural
- restriction fragment length polymorphisms
Definition
A molecular technique that detects genetic variation by comparing the lengths of fragments produced when a is digested with restriction ; differences in fragment patterns reveal sequence at or near enzyme recognition sites.
Etymology
restriction ( cutting) + fragment (piece of ) + length (variable size)
Example
analysis of mitochondrial in bark revealed cryptic previously lumped under one morphological name.
Synonyms
- RFLP analysis
- restriction mapping
Related Terms
- PCR
- microsatellite
- DNA barcoding
- Genetic marker
- population genetics
- haplotype
Usage Notes
Abbreviated , not RELP as sometimes mistyped. Superseded by faster methods (-based markers, sequencing) for most applications, but remains relevant for historical datasets and certain diagnostic assays. Requires relatively large amounts compared to modern alternatives. may reflect point mutations, insertions, deletions, or rearrangements affecting restriction sites.