PCR
- Pronunciation
- /P-C-R/
- Category
- Technique
- Singular
- PCR
- Plural
- PCR
Definition
A method for amplifying DNA by means of DNA polymerases such as Taq DNA polymerase. PCR fundamentally involves denaturing double-stranded DNA, adding dNTPs, DNA polymerase, and primers. DNA synthesis occurs, resulting in a doubling of the number of DNA molecules defined by the primers. Additional rounds of denaturation and synthesis occur, resulting in a geometric increase in DNA molecules because each newly synthesized molecule can serve as the template for subsequent DNA amplification.
Etymology
Acronym for Polymerase Chain Reaction.
Example
PCR is used to amplify specific DNA sequences for identification and analysis.