RAPD-PCR

Pronunciation
/RAPD-PCR/
Category
Molecular Biology Technique
Singular
RAPD-PCR
Plural
RAPD-PCR

Definition

A type of PCR using single primers of arbitrary nucleotide sequence consisting of nine or ten nucleotides with a 50-80% G + C content, and no palindromic sequences. These 10mers can act as a primer for PCR and yield reproducible polymorphisms from random segments of genomic DNA.

Etymology

From the terms 'Random Amplified Polymorphic DNA' (RAPD) and Polymerase Chain Reaction (PCR).

Example

RAPD-PCR is used to generate DNA fingerprints for identifying individuals or populations.

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