PCR-RFLP

Pronunciation
/P-CR R-FLP/
Category
Techniques

Definition

A technique that combines polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. Genomic DNA is amplified by traditional PCR. Once the DNA is amplified, it is cut with restriction enzymes, electrophoresed, and visualized by ethidium bromide staining.

Etymology

Example

PCR-RFLP analysis is used to identify genetic variations between individuals or species.

Synonyms

  • PCR-Restriction Fragment Length Polymorphism

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